Catalog No: OKEH05501
Size:96T
Price: $625.00
SKU
OKEH05501
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ywhaz ELISA Kit (Mouse) (OKEH05501) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ywhaz has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ywhaz is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ywhaz captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 84.3%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.8%, n = 20
Mean Inter-Assay Precision - <11.1%, n = 20
ELISA Kit Component
ComponentAmount
YWHAZ Microplate96 Wells (12 x 8 Well strips)
YWHAZ Lyophilized Standard2
100X Biotinylated YWHAZ Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Mouse 14-3-3 protein zeta/delta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolYwhaz
Gene Full Nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Alias Symbols1110013I11Rik;14-3-3 protein zeta/delta;14-3-3 zeta;14-3-3zeta;AI596267;AL022924;AU020854;KCIP-1;protein kinase C inhibitor protein 1;SEZ-2.
NCBI Gene Id22631
Protein NameP63101
Description of Target14-3-3 protein zeta/delta
Uniprot IDhttps://www.uniprot.org/uniprot/P63101
Protein Accession #NP_001240734.1
Nucleotide Accession #NM_001253805.1
Protein Size (# AA)245
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