YWHAE antibody - middle region (AVARP02058_P050)

Catalog#: AVARP02058_P050

• Size: 50ug

Human Spleen

Human NT-2, mouse brain extracts

• Description of Target: YWHAE is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. YWHAE binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. The binding generally results in the modulation of the activity of the binding partner.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Gene Symbol: YWHAE
• Official Gene Full Name: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Alias Symbols: 14-3-3E; FLJ45465; KCIP-1; MDCR; MDS
• Protein Accession#: NP_006752
• Nucleotide Accession#: NM_006761
• Swissprot Id: P62258
• Host: Rabbit
• Clonality: Polyclonal
• Protein Size (# AA): 255
• Molecular Weight: 29kDa
• Application: WB
• Partner Proteins: ABL1, JUN, NEK1, PIAS2, TSC1, TSC2, ABL1, BAD, BCR, BRAF, CDC25B, CDC25C, CDK16, CDKN1B, CHRNA4, CREBBP, EPB41L2, EPB41L3, ESR1, ESR2, GREM1, ING1, KCNH2, KCNK15, KCNK3, KCNK9, KLC3, KRT18, MAP3K3, MAP3K5, MARK4, MDM4, MED1, MST1R, NCOA1, NCOA2, NCOA3, NEK1, NR3C1, NRIP1, PARD3, PARD6A, PARD6B, PA
• Immunogen: The immunogen for anti-YWHAE antibody: synthetic peptide directed towards the middle region of human YWHAE
• Product Format: Lyophilized powder
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Chicken: 100%; Dog: 100%; Guinea pig: 100%; Mouse: 100%; Zebrafish: 100%; African clawed frog: 92%
• Peptide Sequence: Synthetic peptide located within the following region: ELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDEN
• Blocking Peptide: For anti-YWHAE antibody is Catalog # AAP30642 (Previous Catalog # AAPP01295)
• Key Reference: Tak,H., (2007) Cell. Signal. 19 (11), 2379-2387
• Reconstitution and Storage: Add 50 μl of distilled water. Final anti-YWHAE antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Product Protocols: YWHAE antibody tested with Human Fetal Spleen Tissue (AVARP02058_P050)

Aviva Systems Biology is the original manufacturer of this YWHAE antibody (AVARP02058_P050)

Click here to view the YWHAE antibody Western Blot Protocol

Product Datasheet Link: YWHAE antibody (AVARP02058_P050)

WB Suggested Anti-YWHAE Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Fetal Spleen

Western Blot image:


Description of Target: YWHAE is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. YWHAE binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. The binding generally results in the modulation of the activity of the binding partner.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s YWHAE antibody (AVARP02058_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Review: YWHAE antibody – middle region (AVARP02058_P050) with human NT-2 cells, mouse brain extracts in western blot

YWHAE antibody - middle region (AVARP02058_P050)

Sample Type: 1. Human NT-2 cells (60ug)
2. mouse brain extracts (80ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science

Additional protocol details: 2N HCL 30 min, washed in 3x PBS, stained for 1 hour and 30 minutes, with 1 ug/50 ul antibody and incubated in Alexa goat anti-rabbit 594 for 1 hour. Images taken with a 40x objective.