YWHAB Antibody (OAGA01000)

Catalog No: OAGA01000

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
D: Hep G2
E: Molt-4
F: Raji
12% SDS PAGE
OAGA01000 diluted at 1:10000

Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
OAGA01000 diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta(OAGA01000) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (OAGA01000) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Cytoplasm, Melanosome
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 171 of 14-3-3 beta (Uniprot ID#P31946)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: YWHAB
• Gene Full Name: 14-3-3 protein beta/alpha
• Alias Symbols: HS1, GW128, YWHAA, KCIP-1, HEL-S-1
• NCBI Gene Id: 7529
• Protein Name: 14-3-3 protein beta/alpha
• Uniprot ID: P31946
• Protein Accession #: NP_003395.1
• Molecular Weight: 28 kDa