XRCC5 Antibody (OAGA00808)

Catalog No: OAGA00808

Size:100 ul

Ku80 antibody [N3C2], Internal detects XRCC5 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Ku80 antibody [N3C2], Internal (OAGA00808) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5)(OAGA00808) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 (XRCC5)(OAGA00808) antibody at 1:200 dilution.

Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 ?g of Ku80 antibody (OAGA00808) 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody (OAGA00808) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, IP, WB
• Additional Information: Background: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]
  Positive Controls: 293T, A431, HepG2, A375
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 315 and 593 of Ku80 (Uniprot ID#P13010)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1.01 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: XRCC5
• Gene Full Name: X-ray repair cross-complementing protein 5
• Alias Symbols: KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
• NCBI Gene Id: 7520
• Protein Name: X-ray repair cross-complementing protein 5
• Uniprot ID: P13010
• Protein Accession #: NP_066964.1
• Molecular Weight: 83 kDa