XRCC5 Antibody (OAAB21398)

Catalog No: OAAB21398

Size:100ul

Western blot detection of Ku80 in COS7, Jurkat, Hela, MCF7 and A549 cell lysates using Ku80 mouse mAb (OAAB21398) (1:1,000 diluted). Predicted band size: 86KDa. Observed band size: 86KDa.

Immunofluorescent analysis of Hela cells using Ku80 mouse mAb (OAAB21398) (1:400).

Immunoprecipitation analysis of Hela cell lysates using Ku80 mouse mAb (OAAB21398).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC, IP
• Additional Information: Function: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
  ICC: 1:400
  IP: 1:500
• Reference: Yaneva M.,et al.J. Biol. Chem. 264:13407-13411(1989).
  Mimori T.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:1777-1781(1990).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
  Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: XRCC5
• Gene Full Name: X-ray repair complementing defective repair in Chinese hamster cells 5
• Alias Symbols: KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
• NCBI Gene Id: 7520
• Description of Target: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
• Uniprot ID: P13010
• Protein Accession #: NP_066964.1
• Nucleotide Accession #: NM_021141.3
• Molecular Weight: 83 kDa