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Datasheets/Manuals | Printable datasheet for anti-XRCC5 (OAAB21398) antibody |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid |
Clonality | Monoclonal |
Host | Rabbit |
Application | WB, ICC, IP |
Additional Information | Function: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Predicted Homology Based on Immunogen Sequence | Human, Mouse |
Preservative | 0.02% Sodium Azide and 50% glycerol |
Formulation | Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
Application Info | WB: 1:1000 ICC: 1:400 IP: 1:500 |
Reference | Yaneva M.,et al.J. Biol. Chem. 264:13407-13411(1989). Mimori T.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:1777-1781(1990). Ota T.,et al.Nat. Genet. 36:40-45(2004). Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. |
Gene Symbol | XRCC5 |
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Gene Full Name | X-ray repair complementing defective repair in Chinese hamster cells 5 |
Alias Symbols | KU80, KUB2, Ku86, NFIV, KARP1, KARP-1 |
NCBI Gene Id | 7520 |
Description of Target | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
Uniprot ID | P13010 |
Protein Accession # | NP_066964.1 |
Nucleotide Accession # | NM_021141.3 |
Molecular Weight | 83 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "XRCC5 Antibody (OAAB21398)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "XRCC5 Antibody (OAAB21398)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "XRCC5 Antibody (OAAB21398)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "XRCC5 Antibody (OAAB21398)"?
This target may also be called "KU80, KUB2, Ku86, NFIV, KARP1, KARP-1" in publications.
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What is the shipping cost for "XRCC5 Antibody (OAAB21398)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "XRCC5 Antibody (OAAB21398)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "XRCC5 Antibody (OAAB21398)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "83 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "XRCC5 Antibody (OAAB21398)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "XRCC5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "XRCC5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "XRCC5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "XRCC5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "XRCC5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "XRCC5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.