Catalog No: OKEH01685
Size:96T
Price: $525.00
SKU
OKEH01685
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology WWC1 ELISA Kit (Human) (OKEH01685) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WWC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WWC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WWC1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
WWC1 Microplate96 Wells (12 x 8 Well strips)
WWC1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated WWC1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Protein KIBRA
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolWWC1
Gene Full NameWW and C2 domain containing 1
Alias SymbolsHBeAg-binding protein 3;HBEBP3;HBEBP36;KIBRA;kidney and brain protein;MEMRYQTL;PPP1R168;protein KIBRA;protein phosphatase 1, regulatory subunit 168;protein WWC1;WW domain-containing protein 1;WW, C2 and coiled-coil domain containing 1.
NCBI Gene Id23286
Protein NameQ8IX03
Description of TargetProtein KIBRA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8IX03
Protein Accession #NP_001161661.1
Nucleotide Accession #NM_001161661.1
Protein Size (# AA)1113
Molecular Weight125 kDa
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