Tag Archives: Hela Cells

Product Review: SENP3 antibody – N-terminal region (ARP55322_P050) in HeLa cells using Immunohistochemistry

ShareProduct Page Link: SENP3 antibody – N-terminal region (ARP55322_P050) Data provided by Dr. Michael Matunis of Johns Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix for 30 min RT (2% Formaldehyde in PBS) 3. Wash … Continue reading

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Product Review: NUP35 antibody – N-terminal region (ARP52578_P050) in HeLa cells using Immunohistochemistry

ShareProduct Page Link:  NUP35 antibody – N-terminal region (ARP52578_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 mM … Continue reading

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Product Review: NUP50 antibody – C-terminal region (ARP52288_P050) in HeLa cells using Immunohistochemistry

ShareProduct Page Link:  NUP50 antibody – C-terminal region (ARP52288_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 mM … Continue reading

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Product Review: NUP62 antibody – N-terminal region (ARP46179_P050) in HeLa cells using Immunohistochemistry

ShareProduct Page Link: NUP62 antibody – N-terminal region (ARP46179_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 … Continue reading

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Product Review: NUP98 antibody – N-terminal region (ARP39251_P050) in HeLa cells using Immunohistochemistry

ShareProduct Page Link: NUP98 antibody – N-terminal region (ARP39251_P050) Data provided by Dr. Michael Matunis of John Hopkins University Protocol: 1. Wash HeLa cells 1X with PBS 2. Add fix/perm solution (20 mM PIPES pH=6.8, 4% Formaldehyde, 0.2% TNX100, 10 … Continue reading

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Product Review: GBA antibody – C-terminal region (ARP61322_P050) in rat organs, fibroblasts and HeLa cells using Western Blot.

ShareProduct Page Link: GBA antibody – C-terminal region (ARP61322_P050) Data provided by Dr. Andrea Balreira Protocol: SDS-PAGE and WB Cell culture samples (fibroblasts and HeLa cells) were lysed and rat organs were homogenized, and proteins were quantified by the Bradford … Continue reading

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Product Review: MCM8 antibody – N-terminal region (ARP36658_P050)using Western Blot

ShareProduct Page Link: MCM8 antibody – N-terminal region (ARP36658_P050) Data Provided by Dr. Marcel Mechali Protocol: – primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5% – 3×10 minutes of wash with PBS-Tween 0,1% – secondary antibody … Continue reading

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Product Review: WRNIP1 antibody – N-terminal region (ARP36470_P050) using Western Blot

ShareProduct Page Link: WRNIP1 antibody – N-terminal region (ARP36470_P050) Data Provided by Dr. Marcel Mechali Protocol: – primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5% – 3×10 minutes of wash with PBS-Tween 0,1% – secondary antibody … Continue reading

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Product Review: PMS2 antibody – middle region (ARP56117_P050) in HeLa cells using Western Blot.

ShareProduct Page Link: PMS2 antibody – middle region (ARP56117_P050) Data Provided by Dr. Marcel Mechali Protocol: – primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0,1%-BSA 2,5% – 3×10 minutes of wash with PBS-Tween 0,1% – secondary antibody … Continue reading

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Product Review: CDH1 antibody – middle region (ARP59265_P050) using HeLa whole cell lysate in Western Blots

ShareProduct Page Link: CDH1 antibody – middle region (ARP59265_P050) Data provided by: Dr. Chang, MIT Summary: Cdh1 antibody – specific band at correct molecular weight when used at 1:200 for 1 hour on WB of whole cell lysate. What type of … Continue reading

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