Product Page Link: CTCF Antibody – C-terminal region (ARP31080_P050)
Data Provided by: Dr. Marcel Mechali
- primary antibody over nigth at 4°C at 1:2000 in PBS-Tween 0.2% -fat-free-milk 1%
- 5×10 minutes of wash with PBS-Tween 0,2%
- secondary antibody from GE healthcare (ref NA934V) at 1:3000 in PBS-Tween 0,1%, 1h at room
- 3×10 minutes of wash with PBS-Tween 0,2%
- 20 ug of extract per lane.
Lane 1: CTCF58 Cytoplasm; Lane 2 CTCF58 Nucleoplasm