Product Protocol: Rabbit Anti-SGK1 Antibody (ARP56652_P050) in Human Pineal Tissue using Immunohistochemistry

Aviva Systems Biology is the original manufacturer of this SGK1 antibody.
Product Datasheet Link: SGK1 antibody ARP56652_P050

Rabbit Anti-SGK1 Antibody
Catalog Number: ARP56652_P050
Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec

Left to right:

DAPI, SGK1 Ab, Merge

Control Image:
Control Antibody: Normal Rabbit IgG
Control Antibody Concentration: 1:100

Left to right:

DAPI, Rabbit IgG, Merge

Protocol:

1. Normal adult Human Pineal Tissue was formalin fixed, embedded in paraffin wax, sectioned at 6 micron thickness and put on histological slides.

2. After deparaffinization and rehydration, the low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

3. The blocking buffer was 5% normal goat serum.

4. Primary antibodies was diluted in antibody dilution buffer (1% Normal Donkey Serum) to the final testing dilution (1:100, 1:600, 1:1200).

5. The appropriate anti-rabbit fluorescent-conjugated (Rhodamine:red or FITC:green) secondary antibody was applied and nuclei will be counterstained with DAPI (blue).

6. A Negative control utilized a nonspecific rabbit IgG staining the same normal Human Pineal Tissue.

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