OMA1 antibody cell lysate
Department of Biochemistry
University of Utah SOM
OMA1 ran as expected.
Western Blot Protocol:
Cell lysates were loaded on the protein gel and separated using gel electrophoresis.
After the transfer, the nitrocellulose membrane was blocked in 5% BSA TBST for an hour at RT.
The membrane was incubated overnight at 4C in primary antibody dilution (TBST, 5%BSA with 1:1000 dilution).
The following day the membrane was washed 3X in TBST and blotted in secondary anti-rabbit antibody (TBST, 5%BSA with 1:5000 dilution) for 1 hour at RT.
The membrane was washed 3x in TBST and developed using Western Lightening Chemiluminescence reagent.