|For A Limited Time, 50 ug of anti-His tag Antibody for $45!
Offer valid for Aviva’s monoclonal anti-His tag antibody, clone HIS.H8/EH158, catalog number OAEA00010. Antibody recognizes epitope of 6x His-tag, validated for WB, IF, IP, IS, and ELISA applications.
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Limit 5 vials per customer. All items subject to stock and availability. Discounted items still covered under Aviva’s refund policy. Shipping and handling charges still apply. To view shipping and handling charges click here. Aviva reserves the right to alter or withdraw the any promotion at any time. Any questions about Aviva’s promotions can be addressed to firstname.lastname@example.org.
Please refer to the following references, describing protocols in detail, for Aviva’s anti-His tag antibodies:
Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions
-Using a cell-bound immunogen, generate a monoclonal antibody
-Generate single-chain fragment of variable domains
-Fluorescence-activiated cell sorting analysis
Lindner, P., Bauer, K., et. al. 1997. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. BioTechniques. 22: 140-149
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
-Method for parallel expression and purification of recombinant proteins with His tag or GST tag from bacterial expression systems
-Quick method to purify proteins
-Optimization of the purification protocol
-Reproducibility and protein yield of the purification method
Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies (249 citations)
-Not really methods, more reference
Three-step chromatographic purification procedure for the production of a His-tag recombinant kinesin over expressed in E. Coli
-Western blot using His
-Cell cultures, chromatographic procedures
Gilbert, S., Bakalara, N., Santarelli, X. 2000 Three step chromatographic purification procedure for the production of a His-tag recombinant kinesin overexpressed in E. Coli. J Chromatography. 737(1-2): 143-1