Category Archives: Technical Questions

Technical Questions

What is the sequence of the peptide used to raise this antibody?

ShareAviva has made over 40,000 rabbit polyclonal antibodies using short peptide sequences, usually around 14aa in length.  It is Aviva’s policy not to disclose the sequence of the immunizing peptide.  However, we do offer two indicators of the location of … Continue reading

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Does Aviva Systems Biology have an antibody to [species] [gene name]?

ShareAviva has made over 40,000 rabbit polyclonal antibodies using short peptide sequences, usually around 14aa in length.  Only about half of these antibodies are searchable on our website.  For a complete listing of all the antibodies (validated and unvalidated) that … Continue reading

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Technical Question: What is the origin and make-up of the cut off control reagent?

ShareProduct Page for Rubella IgM Capture ELISA Kit (96 Wells) As I understand it, the wells of the plate are coated with anti-hIgM mAb.  Only a portion (if any) of the bound IgM in a given sample will be anti-Rubella … Continue reading

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Technical Question: Has the COX2 antibody been used successfully with feline COX2?

ShareList of COX2 antibodies Unfortunately, none of our COX-2 antibodies have been used with feline samples yet.  I think the best way to find an antibody that is specific for feline COX-2 would be to use our new antibody BLAST … Continue reading

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Technical Question: Could you please provide reconstitution and storage instructions?

SharePlease see our Reconstitution & Storage Instructions webpage for exactly this kind of information: //www.avivasysbio.com/protocols-procedures/reconstitution-storage-instructions

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Technical Question: What is the concentration of your control human plasma?

ShareProduct Page for Human Prealbumin (Transthyretin) ELISA Kit (96 wells) The range for human plasma may be 0.08 – 0.3 mg/ml

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Technical Question: Does this CHAC1 antibody work in mouse?

ShareProduct Page for CHAC1 antibody – C-terminal region (ARP42623_P050) Unfortunately, this antibody has not been tested with mouse samples.  It does show 100% homology with the mouse CHAC1 sequence (http://www.uniprot.org/uniprot/Q8R3J5) so it’s expected to react.

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Technical Question: What cell types were used to validate the ANO6 antibody in mouse? What was the Western Blot Procedure?

ShareProduct Page for Ano6 antibody – middle region (ARP44759_P050) From the results of our lab, this antibody was tested on mouse and rat brain, heart, kidney, liver, lung, intestine and muscle.  The tissues that showed the correct sized band came from … Continue reading

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Technical Question: Why is there a difference between the predicted and the actual molecular weight for CHRNA1?

ShareProduct Page for CHRNA1 antibody – C-terminal region (AVARP13084_P050) There seems to be a difference between the predicted and actual MW of this protein. My concern is with the molecular weight of the observed band. Several blots I have seen online indicate … Continue reading

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Technical Question: What is the cross reactivity of SHH to DHH and IHH?

ShareProduct Page for SHH antibody – N-terminal region (ARP44235_P050) Based on the sequence of the 14aa immunizing peptide used to generate our anti-SHH antibody (ARP44235_P050), here are the homologies to the protein sequences you have sent: Desert Hedgehog DHH:http://www.ncbi.nlm.nih.gov/protein/O43323.1 57% homology … Continue reading

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