WNT5A Antibody - middle region (OAAB03090)

Catalog No: OAAB03090

Size:200ul

WNT5A Antibody (Center) western blot analysis in Hela cell lysates (35ug/lane).This demonstrates the WNT5A antibody detected the WNT5A protein (arrow).

WNT5A is strongly supported by BioGPS gene expression data to be expressed in Hela

WNT5A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT5A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

WNT5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Rabbit
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC
• Additional Information: Cellular Location:  Secreted, extracellular space, extracellular matrix
  Tissue Location: Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable Expression is found in thyrocytes but not in stromal cells (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human WNT5A.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rabbit; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  WNT5A is strongly supported by BioGPS gene expression data to be expressed in Hela

• Application Info: FC: 1:25
  WB: 1:500
  WB: 1:2000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: WNT5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: WNT5A
• Alias Symbols: hWNT5A
• NCBI Gene Id: 7474
• Protein Name: Protein Wnt-5a
• Uniprot ID: P41221
• Protein Accession #: NP_003383.2
• Molecular Weight: 42339 Da