WNT4 Antibody (OAGA01465)

Catalog No: OAGA01465

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: WNT4 transfected 293T lysates
10% SDS PAGE
OAGA01465 diluted at 1:5000

WNT4 antibody detects WNT4 protein by western blot analysis. Non-transfected (-) and Wnt4-transfected (+, including V5-tag) 293T whole cell extracts (100 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (OAGA01465) at a dilution of 1:2500.
The V5 was used as internal control shown at the bottom panel.

WNT4 antibody detects WNT4 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (OAGA01465) diluted at a dilution of 1:1000.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using WNT4 (OAGA01465) antibody at 1:500 dilution.

WNT4 antibody detects WNT4 protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNT4 antibody (OAGA01465) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human WNT4. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.78mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:10000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: WNT4
• Gene Full Name: wingless-type MMTV integration site family, member 4
• Alias Symbols: WNT-4, SERKAL
• NCBI Gene Id: 54361
• Description of Target: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.
• Uniprot ID: P56705