WNT3A High Sensitivity ELISA Kit (Human) (OKCD01389)

Catalog No: OKCD01389

Size:96WELLS

Human WNT3A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human|Mouse|Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-93%	82-98%	78-102%	81-92%
  EDTA Plasma (n=5)	90-104%	80-90%	92-104%	84-92%
  Heparin Plasma (n=5)	80-98%	81-91%	93-103%	78-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Wingless Type MMTV Integration Site Family, Member 3A (WNT3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-99	96
  EDTA Plasma (n=5)	89-101	92
  Heparin Plasma (n=5)	87-95	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-WNT3A Microplate: 96 Wells (12 x 8 Well Strips)
  • WNT3A Lyophilized Standard: 2
  • 100X Biotinylated WNT3A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: WNT3A
• Gene Full Name: Wnt family member 3A
• Alias Symbols: protein Wnt-3a;wingless-type MMTV integration site family, member 3A.
• NCBI Gene Id: 89780
• Protein Name: Protein Wnt-3a
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
• Uniprot ID: P56704
• Protein Accession #: NP_149122.1
• Nucleotide Accession #: NM_033131.3