Catalog No: OKEH00578
Size:96T
Price: $425.00
SKU
OKEH00578
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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WFDC2 ELISA Kit (Human) : 96 Wells (OKEH00578)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology WFDC2 ELISA Kit (Human) (OKEH00578) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human WFDC2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human WFDC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human WFDC2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.7% (n = 20)
Mean Inter-assay CV%: < 10.1% (n = 20)
ELISA Kit Component
ComponentAmount
WFDC2 Microplate96 Wells (12 x 8 Well strips)
WFDC2 Lyophilized Standard2
100X Biotinylated WFDC2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human WAP four-disulfide core domain protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolWFDC2
Gene Full NameWAP four-disulfide core domain 2
Alias SymbolsdJ461P17.6;EDDM4;epididymal protein 4;epididymal secretory protein E4;epididymis secretory sperm binding protein;epididymis-specific, whey-acidic protein type, four-disulfide core;HE4;human epididymis protein 4;major epididymis-specific protein E4;putative protease inhibitor WAP5;WAP domain containing protein HE4-V4;WAP four-disulfide core domain protein 2;WAP5.
NCBI Gene Id10406
Protein NameQ14508
Description of TargetWAP four-disulfide core domain protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q14508
Protein Accession #NP_006094.3
Nucleotide Accession #NM_006103.3
Protein Size (# AA)124
Molecular Weight13 kDa
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