WASL Antibody - middle region (ARP63498_P050)

Catalog No: ARP63498_P050

WB Suggested Anti-WASL Antibody
Titration: 1.0 ug/ml
Positive Control: Fetal Lung

Host: Mouse
Target Name: WASL
Sample Tissue: Mouse Skeletal Muscle
Antibody Dilution: 1ug/ml

Rabbit Anti-WASL Antibody
Catalog Number: ARP63498_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: ISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-WASL (ARP63498_P050) antibody is Catalog # AAP63498

Target Info

• Gene Symbol: WASL
• Gene Full Name: Wiskott-Aldrich syndrome-like
• Alias Symbols: NWASP, WASPB, N-WASP
• NCBI Gene Id: 8976
• Protein Name: Neural Wiskott-Aldrich syndrome protein
• Description of Target: The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.
• Uniprot ID: O00401
• Protein Accession #: NP_003932
• Nucleotide Accession #: NM_003941
• Protein Size (# AA): 505
• Molecular Weight: 56kDa
• Protein Interactions: RHOJ; ARPC3; RNF8; NCK2; WIPF1; SDCBP; PTK6; GRB2; IQGAP1; WWOX; SNX9; APBB1; PRPF40A; FNBP1L; FNBP1; TRIP10; PSTPIP1; ACTG1; SORBS3; ACTR2; ACTR3; LCK; CDC42; RPL7AP66; ZNF395; BAIAP2; ARPC1B; FCHSD2; IPO5; HP; HSP90AB1; HSP90AA1; SRC; WASF2; APP; WIPF2;