VRK1 Antibody - middle region (OAAB17053)

Catalog No: OAAB17053

Size:400ul

The anti-VRK1 Pab (Cat. # OAAB17053) is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).

Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381) (Cat. # OAAB17053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene.

VRK1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, HL60

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Precautions: VRK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3 Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm
  Cellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle (By similarity). Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis
  Tissue Location: Widely expressed. Highly expressed in fetal liver, testis and thymus
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-396 amino acids from the Central region of human VRK1.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  VRK1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, HL60

• Application Info: WB: 1:2000

Target Info

• Gene Symbol: VRK1
• Alias Symbols: PCH1, PCH1A
• NCBI Gene Id: 7443
• Protein Name: Serine/threonine-protein kinase VRK1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q99986
• Protein Accession #: NP_003375.1
• Molecular Weight: 45476 Da