VPS29 Antibody (OAEB00843)

Catalog No: OAEB00843 (Formerly GWB-68848C)

Size:100UG

OAEB00843 (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Additional Information: Epitope Info: internal region
  Immunogen: Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence according to NP_057310.1; NP_476528.1.
  Note: This antibody is expected to recognise both reported isoforms (NP_057310.1 and NP_476528.1).
  Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: VPS29
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: CTGNLCTKESYDYLK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:16000.
  Immunohistochemistry: In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands. Recommended concentration, 4-8ug/ml.
  Western Blot: Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: Nakada-Tsukui K, Saito-Nakano Y, Ali V, Nozaki T.
  A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica.
  Mol Biol Cell. 2005 Nov;16(11):5294-303. Epub 2005 Aug 24. PMID: 16120649

Target Info

• Gene Symbol: Vps29|VPS29
• Gene Full Name: VPS29 retromer complex component
• Alias Symbols: 2010015D08Rik;AW049835;DC15;DC7;epididymis secretory sperm binding protein;hVPS29;PEP;PEP11;PEP11 homolog;retromer protein;vacuolar protein sorting 29;vacuolar protein sorting 29 homolog;vacuolar protein sorting-associated protein 29;vacuolar sorting protein 29;vacuolar sorting protein VPS29/PEP11;vesicle protein sorting 29;x 007 protein.
• NCBI Gene Id: 288666|51699|56433
• Protein Name: Vacuolar protein sorting-associated protein 29
• Uniprot ID: Q9UBQ0
• Protein Accession #: NP_057310.1
• Molecular Weight: 20.5; 20.9