VPS25 Antibody (OAEB02342)

Catalog No: OAEB02342 (Formerly GWB-91DE13)

Size:100UG

OAEB02342 (0.5ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02342 (10ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: VPS25
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: C-QPNVDTRQKQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:1000.
  Western Blot: Approx 20-22kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 ug/ml.
  Immunohistochemistry: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Recommended concentration, 10ug/ml.
• Reference: * Vaccari T, Bilder D.; The Drosophila Tumor Suppressor vps25 Prevents Nonautonomous Overproliferation by Regulating Notch Trafficking.; Dev Cell. 2005 Nov;9(5):687-98.; Pubmed 16256743

Target Info

• Gene Symbol: VPS25
• Gene Full Name: vacuolar protein sorting 25 homolog
• Alias Symbols: dermal papilla-derived protein 9;DERP9;EAP20;ELL-associated protein of 20 kDa;ESCRT-II complex subunit VPS25;FAP20;vacuolar protein-sorting-associated protein 25.
• NCBI Gene Id: 84313
• Protein Name: Vacuolar protein-sorting-associated protein 25
• Uniprot ID: Q9BRG1
• Protein Accession #: NP_115729.1
• Molecular Weight: 20.7