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Catalog No: OKEH03132
Size:96T
Price: $525.00
SKU
OKEH03132
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology VKORC1 ELISA Kit (Rat) (OKEH03132) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vkorc1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Vkorc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vkorc1 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
Anti-Vkorc1 Microplate96 Wells (12 x 8 Well strips)
Vkorc1 Lyophilized Standard2
100X Biotinylated Vkorc1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.318 ng/mL
SpecificityNatural and recombinant Rat Vitamin K epoxide reductase complex subunit 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVkorc1
Gene Full Namevitamin K epoxide reductase complex, subunit 1
Alias Symbolsphylloquinone epoxide reductase;vitamin K epoxide reductase complex subunit 1;vitamin K1 2,3-epoxide reductase subunit 1;vitamin K1 epoxide reductase (warfarin-sensitive);Warfarin resistance.
NCBI Gene Id309004
Protein NameQ6TEK4
Description of TargetVitamin K epoxide reductase complex subunit 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q6TEK4
Protein Accession #NP_976080.1
Nucleotide Accession #NM_203335.2
Protein Size (# AA)161
Molecular Weight18 kDa
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