VIP ELISA Kit (Human) : 96 Wells (OKEH00406)

Catalog No: OKEH00406

Size:96T

Human VIP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology VIP ELISA Kit (Human) (OKEH00406) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human VIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human VIP and incubated. The Human VIP found in the sample or standards competes with the biotinylated Human VIP for limited binding sites on the immobilized anti-Human VIP antibody. Excess unbound biotinylated Human VIP and sample or standard human VIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human VIP captured in the well and inversely proportional to the amount of human VIP which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.8% (n = 20)
• ELISA Kit Component:
  • Anti-VIP Microplate: 96 Wells (12 x 8 Well strips)
  • VIP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X VIP-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.78 pg/mL
• Specificity: Natural and recombinant Human VIP peptides
• Publications:

  ER Lipid Defects in Neuropeptidergic Neurons Impair Sleep Patterns in Parkinson's Disease. Neuron. 98, 1155-1169.e6 (2018). 29887339

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for VIP ELISA Kit (Human) : 96 Wells (OKEH00406) on CiteAb

Target Info

• Gene Symbol: VIP
• Gene Full Name: vasoactive intestinal peptide
• Alias Symbols: PHM27;prepro-VIP;VIP peptides.
• NCBI Gene Id: 7432
• Protein Name: P01282
• Description of Target: VIP peptides
• Uniprot ID: https://www.uniprot.org/uniprot/P01282
• Protein Accession #: NP_003372.1
• Nucleotide Accession #: NM_003381.3
• Protein Size (# AA): 170
• Molecular Weight: 19 kDa