VIM ELISA Kit (Mouse) (OKCD02979)

Catalog No: OKCD02979

Size:96WELLS

Mouse VIM ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-96%	89-103%	83-90%	80-90%
  EDTA Plasma (n=5)	83-105%	91-101%	80-98%	80-104%
  Heparin Plasma (n=5)	82-89%	85-94%	79-101%	90-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vimentin (VIM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vimentin (VIM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vimentin (VIM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vimentin (VIM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-105	98
  EDTA Plasma (n=5)	80-99	89
  Heparin Plasma(n=5)	81-102	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vimentin (VIM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vimentin (VIM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-VIM Microplate: 96 Wells (12 x 8 Well strips)
  • VIM Lyophilized Standard: 2
  • 100X Biotinylated VIM Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vimentin (VIM). No significant cross-reactivity or interference between Vimentin (VIM) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Vim
• Gene Full Name: vimentin
• Alias Symbols: vimentin.
• NCBI Gene Id: 22352
• Protein Name: Vimentin
• Description of Target: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
• Uniprot ID: P20152
• Protein Accession #: NP_035831.2
• Nucleotide Accession #: NM_011701.4
• Protein Size (# AA): 466