VIM Antibody (OAEE00561)

Catalog No: OAEE00561

Size:100UG

Immunofluorescence staining of chicken postnatal erythrocytes.
Tubulin (red) was stained with anti-alpha-tubulin (TU-01), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa.
Lr = lysate (reducing conditions)
Lnr = lysate (non-reducing conditions)
IPr = immunoprecipitate (reducing conditions)
IPnr = immunoprecipitate (non-reducing conditions)

Product Info

• Predicted Species Reactivity: Chicken|Human|Mouse|Porcine|Rat
• Product Format: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: VI-10
• Isotype: IgM
• Host: Mouse
• Application: Immunocytochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.
• Formulation: Purified
• Application Info: ICC: Staining technique: a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
  Positive Control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
  IHC-P: 5 ug/mL. Positive Tissue: Skin fibroblast.

Target Info

• Gene Symbol: VIM
• Gene Full Name: vimentin
• Alias Symbols: epididymis secretory sperm binding protein;vimentin.
• NCBI Gene Id: 7431
• Protein Name: Vimentin
• Description of Target: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
• Uniprot ID: P08670
• Protein Accession #: NP_003371.2; XP_006717563.1
• Nucleotide Accession #: NM_003380.3; XM_006717500.1
• Molecular Weight: 57 kDa