VIM Antibody (OAEC04472)

Catalog No: OAEC04472

Size:100 ug/100 ul

Product Info

• Predicted Species Reactivity: Human
• Product Format: Ascitic fluid containing 0.03% sodium azide
• Clonality: Monoclonal
• Clone: 4F2E9
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of Vim entin (aa2-466) expres sed in EColi.
• Concentration: 1 mg/ml
• Application Info: WB: 1/500 - 1/2000
  IHC: 1/200 - 1/1000
  ELISA: 1/10000
  

Target Info

• Gene Symbol: VIM
• Gene Full Name: vimentin
• Alias Symbols: HEL113, CTRCT30
• NCBI Gene Id: 7431
• Description of Target: Vim entin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchym al cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vim entin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vim entin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myos in light chain dependent contractile event. This results in cytos keletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vim entin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lym phomas and vas cular tumors may all stain for Vim entin. Vim entin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.
• Uniprot ID: P08670
• Molecular Weight: 54 kDa