Catalog No: OKCD02977
Size:96WELLS
Price: $725.00
SKU
OKCD02977
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for VHL ELISA Kit (Rat) (OKCD02977) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Hippel Lindau Tumor Suppressor (vHL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Von Hippel Lindau Tumor Suppressor (vHL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Hippel Lindau Tumor Suppressor (vHL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Von Hippel Lindau Tumor Suppressor (vHL) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Hippel Lindau Tumor Suppressor (vHL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Hippel Lindau Tumor Suppressor (vHL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.113 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Von Hippel Lindau Tumor Suppressor (vHL). No significant cross-reactivity or interference between Von Hippel Lindau Tumor Suppressor (vHL) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | Vhl |
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Gene Full Name | von Hippel-Lindau tumor suppressor |
Alias Symbols | pVHL;Vhlh;von Hippel-Lindau disease tumor suppressor;von Hippel-Lindau protein;von Hippel-Lindau syndrome homolog;von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase. |
NCBI Gene Id | 24874 |
Protein Name | von Hippel-Lindau disease tumor suppressor |
Description of Target | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2 (By similarity). |
Uniprot ID | Q64259 |
Protein Accession # | NP_434688.1 |
Nucleotide Accession # | NM_052801.1 |
Protein Size (# AA) | 185 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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