VHL ELISA Kit (Human) : 96 Wells (OKEH01354)

Catalog No: OKEH01354

Size:96T

Human VHL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology VHL ELISA Kit (Human) (OKEH01354) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VHL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VHL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VHL captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 9.7% (n = 20)
• ELISA Kit Component:
  • VHL Microplate: 96 Wells (12 x 8 Well strips)
  • VHL Lyophilized Standard: 2
  • 100X Biotinylated VHL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Von Hippel-Lindau disease tumor suppressor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: VHL
• Gene Full Name: von Hippel-Lindau tumor suppressor
• Alias Symbols: elongin binding protein;HRCA1;protein G7;pVHL;RCA1;VHL1;von Hippel-Lindau disease tumor suppressor;von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase.
• NCBI Gene Id: 7428
• Protein Name: P40337
• Description of Target: von Hippel-Lindau disease tumor suppressor
• Uniprot ID: https://www.uniprot.org/uniprot/P40337
• Protein Accession #: NP_000542.1
• Nucleotide Accession #: NM_000551.3
• Protein Size (# AA): 213
• Molecular Weight: 24 kDa