VEGFR2/KDR ELISA Kit (Human) (OKBB00275)

Catalog No: OKBB00275

Size:96 Tests

Human KDR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR2 has been precoated onto 96-well plates. Standards(NS0, A20-E764) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human VEGFR2 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	197	1466	2458	234	1630	2863
  St. Dev.	9.65	51.31	127.8	17.55	114.1	177.5
  %CV	4.89	3.5	5.19	7.5	7	6.19

• ELISA Kit Component:
  • Lyophilized recombinant human KDR standard: 10 ng/tube x 2
  • Anti-human KDR Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human KDR antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 62.5pg/ml-4000pg/ml
  Principle: Aviva’s human VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human VEGFR2 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGFR2 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <4 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Shay-Salit, A., Shushy, M., Wolfovitz, E., Yahav, H., Breviario, F., Dejana, E., Resnick, N. VEGF receptor 2 and the
  adherens junction as a mechanical transducer in vascular endothelial cells. Proc. Nat. Acad. Sci. 99: 9462-9467, 2002.
  2. Sait, S. N. J., Dougher-Vermazen, M., Shows, T. B., Terman, B. I. The kinase insert domain receptor gene (KDR) has
  been relocated to chromosome 4q11-q12. Cytogenet. Cell Genet. 70: 145-146, 1995.
• Specificity: Natural and recombinant human VEGFR2
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A20-E764
• Description: For quantitative detection of human VEGFR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: KDR
• Gene Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Alias Symbols: CD309, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), Protein tyrosine kinase receptor Flk 1, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR 2, VEGFR2
• NCBI Gene Id: 3791
• Protein Name: Vascular endothelial growth factor receptor 2
• Description of Target: Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
• Uniprot ID: P35968
• Protein Accession #: NP_002244.1
• Nucleotide Accession #: NM_002253.2