VCP Antibody (OAGA00993)

Catalog No: OAGA00993

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
C: Molt-4
D: Raji
7.5% SDS PAGE
OAGA00993 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
OAGA00993 diluted at 1:5000

VCP antibody detects VCP protein by Western blot analysis.
A. 50 ug rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (OAGA00993) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(OAGA00993) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(OAGA00993) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain, rat brain
  Cellular Localization: Cytoplasm, cytosol, Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID#P55072)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: VCP
• Gene Full Name: Transitional endoplasmic reticulum ATPase
• Alias Symbols: p97, TERA, CDC48, FTDALS6
• NCBI Gene Id: 7415
• Protein Name: Transitional endoplasmic reticulum ATPase
• Uniprot ID: P55072
• Protein Accession #: NP_009057.1
• Molecular Weight: 89 kDa