USP47 Antibody (OAGA00470)

Catalog No: OAGA00470

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
USP47 antibody
OAGA00470 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
OAGA00470 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using USP47(OAGA00470) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues.
  Positive Controls: A549, HeLa, HepG2, HCT116, mouse brain
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 635 and 870 of USP47
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.82 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: USP47
• Gene Full Name: Ubiquitin carboxyl-terminal hydrolase 47
• Alias Symbols: TRFP
• NCBI Gene Id: 55031
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 47
• Uniprot ID: Q96K76
• Protein Accession #: NP_060414.3
• Molecular Weight: 147 kDa