USP21 Antibody - C-terminal region (OAAB15763)

Catalog No: OAAB15763

Size:400ul

Western blot analysis of anti-USP21 Antibody (C-term) (Cat. # OAAB15763) in T47D cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Cow
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: USP21 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity) Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates
  Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human USP21.
• Predicted Homology Based on Immunogen Sequence: Cow; Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000
  WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: USP21
• Alias Symbols: USP16, USP23
• NCBI Gene Id: 27005
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 21
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9UK80
• Protein Accession #: NP_001014443.1
• Molecular Weight: 62656 Da