USP20 Antibody - middle region (ARP59314_P050)

Catalog No: ARP59314_P050

WB Suggested Anti-USP20 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human heart

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human USP20
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 85%; Rabbit: 100%; Rat: 92%
• Peptide Sequence: Synthetic peptide located within the following region: PAEAQPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPP
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-USP20 (ARP59314_P050) antibody is Catalog # AAP59314 (Previous Catalog # AAPP45364)

Target Info

• Gene Symbol: USP20
• Gene Full Name: Ubiquitin specific peptidase 20
• Alias Symbols: VDU2, hVDU2, LSFR3A
• NCBI Gene Id: 10868
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 20
• Description of Target: USP20 is a deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling.USP20 acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). USP20 plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. USP20 deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity.USP20 mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
• Uniprot ID: Q9Y2K6
• Protein Accession #: NP_006667
• Nucleotide Accession #: NM_006676
• Protein Size (# AA): 913
• Molecular Weight: 102kDa
• Protein Interactions: MDFI; MAGEA11; TRIM23; ALAS1; FATE1; BEND5; SPRY2; VAPB; CCDC36; BEND7; RAD17; NEURL4; USP33; USP20; CCP110; ADRB2; PJA1; USP28; USP21; USP15; PLA2G2A; UBC; VHL; HIF1A; VAPA; HERC2P2; PLEKHA7; SYTL4; GPT2; POLDIP3; RGPD5; ATAD3B; MAP7D1; LRRC59; ZNF770; S