URGCP ELISA Kit (Mouse) (OKCD00613)

Catalog No: OKCD00613 (Formerly GWB-KBBVP7)

Size:96WELLS

Mouse Urgcp ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	85-94%	84-92%	99-105%	92-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Upregulator Of Cell Proliferation (URGCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Upregulator Of Cell Proliferation (URGCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Upregulator Of Cell Proliferation (URGCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Upregulator Of Cell Proliferation (URGCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	83-97	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Upregulator Of Cell Proliferation (URGCP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Upregulator Of Cell Proliferation (URGCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Urgcp Microplate: 96 Wells (12 x 8 Well Strips)
  • Urgcp Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Urgcp Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Upregulator Of Cell Proliferation (URGCP). No significant cross-reactivity or interference between Upregulator Of Cell Proliferation (URGCP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Urgcp
• Gene Full Name: upregulator of cell proliferation
• Alias Symbols: 2010005J08Rik;9130001I21Rik;AW060359;HBV X protein up-regulated gene 4 protein homolog;HBxAg up-regulated gene 4 protein homolog;mKIAA1507;protein URG4;up-regulator of cell proliferation;Urg4.
• NCBI Gene Id: 72046
• Protein Name: Up-regulator of cell proliferation
• Description of Target: May be involved in cell cycle progression through the regulation of cyclin D1 expression.
• Uniprot ID: Q5NCI0
• Protein Accession #: NP_001071129
• Nucleotide Accession #: NM_001077661.1
• Protein Size (# AA): 926