- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-UNC13B antibody - OAGA00889 |
---|
Predicted Species Reactivity | Human |
---|---|
Product Format | Liquid |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | ICC, IF, IHC-FFPE, WB |
Additional Information | Background: This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq] |
:: | Positive Controls: 293T, H1299, HeLa, HepG2 |
:: | Cellular Localization: Cytoplasm (By similarity), Membrane; Peripheral membrane protein (By similarity), Cell membrane (By similarity), Cell junction, synapse (By similarity) |
Reconstitution and Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Immunogen | Recombinant fragment corresponding to a region within amino acids 680 and 957 of UNC13B (Uniprot ID#O14795) |
Purification | Purified by antigen-affinity chromatography. |
Concentration | 1 mg/ml |
Application Info | Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Gene Symbol | UNC13B |
---|---|
Gene Full Name | Protein unc-13 homolog B |
Alias Symbols | UNC13, MUNC13, Unc13h2, munc13-2 |
NCBI Gene Id | 10497 |
Protein Name | Protein unc-13 homolog B |
Uniprot ID | O14795 |
Protein Accession # | NP_006368.3 |
Molecular Weight | 181 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "UNC13B Antibody (OAGA00889)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
-
How long will it take to receive "UNC13B Antibody (OAGA00889)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "UNC13B Antibody (OAGA00889)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UNC13B Antibody (OAGA00889)"?
This target may also be called "UNC13, MUNC13, Unc13h2, munc13-2" in publications.
-
What is the shipping cost for "UNC13B Antibody (OAGA00889)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "UNC13B Antibody (OAGA00889)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "UNC13B Antibody (OAGA00889)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "181 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UNC13B Antibody (OAGA00889)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "UNC13B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "UNC13B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "UNC13B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "UNC13B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "UNC13B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "UNC13B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.