UGT1A9 Antibody (OAGA01064)

Catalog No: OAGA01064

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
UGT1A9 antibody
OAGA01064 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
OAGA01064 diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded Hepatoma, using UGT1A9(OAGA01064) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols. [provided by RefSeq]
  Positive Controls: H1299, HeLa, HepG2, mouse liver
  Cellular Localization: Microsome, Endoplasmic reticulum membrane; Single-pass membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 291 and 530 of UGT1A9 (Uniprot ID#O60656)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.47 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: UGT1A9
• Gene Full Name: UDP-glucuronosyltransferase 1-9
• Alias Symbols: LUGP4, UDPGT, UGT1I, HLUGP4, UGT-1I, UGT1-9, UGT1.9, UGT1AI, UGT1-09, UGT1A9S, UDPGT 1-9
• NCBI Gene Id: 54600
• Protein Name: UDP-glucuronosyltransferase 1-9
• Uniprot ID: O60656
• Protein Accession #: NP_066307.1
• Molecular Weight: 60 kDa