Catalog No: OKEH03446
Size:96T
Price: $525.00
SKU
OKEH03446
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology UCP1 ELISA Kit (Rat) (OKEH03446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ucp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ucp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ucp1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.091 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat Mitochondrial brown fat uncoupling protein 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Ucp1 |
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Gene Full Name | uncoupling protein 1 |
Alias Symbols | mitochondrial brown fat uncoupling protein 1;mitochondrial brown fat uncoupling protein 1-like;solute carrier family 25 member 7;thermogenin;Ucp;UCP 1;Ucpa;uncoupling protein 1 (mitochondrial, proton carrier);Uncp. |
NCBI Gene Id | 24860 |
Protein Name | P04633 |
Description of Target | Mitochondrial brown fat uncoupling protein 1 |
Uniprot ID | https://www.uniprot.org/uniprot/P04633 |
Protein Accession # | NP_036814.1 |
Nucleotide Accession # | NM_012682.2 |
Protein Size (# AA) | 307 |
Molecular Weight | 33 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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