UCN3 ELISA Kit (Mouse) (OKCD02969)

Catalog No: OKCD02969

Size:96WELLS

UCN3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-99%	92-105%	86-94%	78-101%
  EDTA Plasma (n=5)	83-97%	82-101%	86-99%	95-102%
  Heparin Plasma (n=5)	97-105%	85-94%	97-104%	97-104%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Urocortin 3 (UCN3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Urocortin 3 (UCN3) and unlabeled Urocortin 3 (UCN3) (Standards or samples) with the pre-coated antibody specific to Urocortin 3 (UCN3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Urocortin 3 (UCN3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Urocortin 3 (UCN3) in the sample.
• ELISA Kit Range: 12.35-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-102	97
  EDTA Plasma (n=5)	81-99	91
  Heparin Plasma (n=5)	88-99	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urocortin 3 (UCN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urocortin 3 (UCN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ucn3 Microplate: 96 Wells (12 x 8 Well strips)
  • Ucn3 Lyophilized Standard: 2 x 1 ng
  • 100X Ucn3 Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 4.82 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Urocortin 3 (UCN3). No significant cross-reactivity or interference between Urocortin 3 (UCN3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: Ucn3
• Gene Full Name: urocortin 3
• Alias Symbols: ucn III;Urocortin III;urocortin-3.
• NCBI Gene Id: 83428
• Protein Name: Urocortin-3
• Description of Target: Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress (By similarity).
• Uniprot ID: Q924A4
• Protein Accession #: NP_112540.2
• Nucleotide Accession #: NM_031250.5
• Molecular Weight: 164 kDa