UCN ELISA Kit (Rat) (OKCD02957)

Catalog No: OKCD02957

Size:96WELLS

Rat UCN ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-102%	80-101%	81-102%	98-105%
  EDTA Plasma (n=5)	91-105%	96-105%	96-104%	81-103%
  Heparin Plasma (n=5)	78-92%	79-104%	93-105%	80-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urocortin (UCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Urocortin (UCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urocortin (UCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Urocortin (UCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-90	86
  EDTA Plasma (n=5)	95-103	98
  Heparin Plasma(n=5)	91-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urocortin (UCN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urocortin (UCN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-UCN Microplate: 96 Wells (12 x 8 Well strips)
  • UCN Lyophilized Standard: 2
  • 100X Biotinylated UCN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 5.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Urocortin 1 (UCN1). No significant cross-reactivity or interference between Urocortin 1 (UCN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ucn
• Gene Full Name: urocortin
• Alias Symbols: corticotensin;urocortin.
• NCBI Gene Id: 29151
• Protein Name: Urocortin
• Description of Target: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) (PubMed:7477349). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta (By similarity). Plays a role in the establishment of normal hearing thresholds (By similarity). Reduces food intake and regulates ghrelin levels in gastric body and plasma (PubMed:21540451).
• Uniprot ID: P55090
• Protein Accession #: NP_062023.1
• Nucleotide Accession #: NM_019150.1
• Protein Size (# AA): 122