UBR5 Antibody (OAAF01950)

Catalog No: OAAF01950 (Formerly GWB-ASD220)

Size:100 ug

Western blot analysis of extracts from A549 cells, using EDD Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EDD Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human EDD.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: EDD antibody detects endogenous levels of total EDD protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Michelle J. Henderson, J. Biol. Chem., Dec 2006; 281: 39990 - 40000.
  Lisa N. Kinch, Protein Sci., Feb 2005; 14: 360 - 367.
  Michelle J. Henderson, J. Biol. Chem., Jul 2002; 277: 26468 - 26478.
  Darren N. Saunders, Mol. Cell. Biol., Aug 2004;

Target Info

• Gene Symbol: UBR5
• Gene Full Name: ubiquitin protein ligase E3 component n-recognin 5
• Alias Symbols: DD5;E3 identified by differential display;E3 ubiquitin-protein ligase UBR5;E3 ubiquitin-protein ligase, HECT domain-containing 1;EDD;EDD1;HECT-type E3 ubiquitin transferase UBR5;HYD;hyperplastic discs protein homolog;progestin-induced protein.
• NCBI Gene Id: 51366
• Protein Name: E3 ubiquitin-protein ligase UBR5
• Description of Target: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.
• Uniprot ID: O95071
• Protein Accession #: NP_056986.2
• Molecular Weight: 309 kDa