UBE2W Antibody - C-terminal region (OAAB09908)

Catalog No: OAAB09908

Size:400ul

UBE2W Antibody (C-term) (Cat. #OAAB09908) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow)

UBE2W Antibody (C-term) (Cat. #OAAB09908) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).

UBE2W Antibody (C-term) (Cat. #OAAB09908)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Zebrafish
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: UBE2W Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination
  Cellular Location: Nucleus. Note=In the nucleus, colocalizes with FANCL
  Tissue Location: Widely expressed, with highest expression in brain, liver, pancreas and heart
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat; Zebrafish
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: UBE2W
• Alias Symbols: UBC16, UBC-16
• NCBI Gene Id: 55284
• Protein Name: Ubiquitin-conjugating enzyme E2 W
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q96B02
• Protein Accession #: NP_001001481.2
• Molecular Weight: 17331 Da