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Catalog No: OKEH04399
Size:96T
Price: $525.00
SKU
OKEH04399
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology UBE2A ELISA Kit (Human) (OKEH04399) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UBE2A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for UBE2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UBE2A captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.1% (n = 20)
Mean Inter-assay CV%: <= 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-UBE2A Microplate96 Wells (12 x 8 Well Strips)
UBE2A Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
UBE2A Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Ubiquitin-conjugating enzyme E2 A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolUBE2A
Gene Full Nameubiquitin conjugating enzyme E2 A
Alias SymbolsE2 ubiquitin-conjugating enzyme A;HHR6A;MRXS30;MRXSN;RAD6 homolog A;RAD6A;UBC2;ubiquitin carrier protein A;ubiquitin-conjugating enzyme E2 A;ubiquitin-conjugating enzyme E2A;ubiquitin-protein ligase A.
NCBI Gene Id7319
Protein NameP49459
Description of TargetUbiquitin-conjugating enzyme E2 A
Uniprot IDhttps://www.uniprot.org/uniprot/P49459
Protein Accession #NP_001269090.1
Nucleotide Accession #NM_001282161.1
Protein Size (# AA)152
Molecular Weight17 KDa
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