UBA52 Antibody (OAAF01979)

Catalog No: OAAF01979 (Formerly GWB-ASD248)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Ubiquitin Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Ubiquitin.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Ubiquitin antibody detects endogenous levels of total ubiquitin protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:20000
• Reference: John Hanna, Mol. Cell. Biol., Dec 2003; 23: 9251 - 9261.
  Matthias Majetschak, Blood, Mar 2003; 101: 1882 - 1890.
  Fu Shang, J. Biol. Chem., May 2005; 280: 20365 - 20374.
  Joerg Herrmann, Circ. Res., May 2007; 100: 1276 - 1291.

Target Info

• Gene Symbol: RPS27A|UBA52|UBB|UBC
• Gene Full Name: ribosomal protein S27a|ubiquitin A-52 residue ribosomal protein fusion product 1|ubiquitin B|ubiquitin C
• Alias Symbols: 40S ribosomal protein S27a;CEP52;CEP80;epididymis luminal protein 112;epididymis secretory protein Li 50;HEL112;HEL-S-50;HMG20;HUBCEP52;L40;polyubiquitin B;polyubiquitin-B;polyubiquitin-C;RPL40;S27A;UBA80;UBC;UBCEP1;UBCEP80;Ubiquitin A-52 residue ribosomal protein fusion product 1;ubiquitin and ribosomal protein S27a;ubiquitin C;ubiquitin carboxyl extension protein 52;ubiquitin carboxyl extension protein 80;ubiquitin-40S ribosomal protein S27a;ubiquitin-52 amino acid fusion protein;ubiquitin-60S ribosomal protein L40;ubiquitin-CEP52;ubiquitin-CEP80.
• NCBI Gene Id: 6233|7311|7314|7316
• Protein Name: Polyubiquitin-B|Polyubiquitin-C|Ubiquitin-40S ribosomal protein S27a|Ubiquitin-60S ribosomal protein L40
• Description of Target: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
• Uniprot ID: P0CG47|P0CG48|P62979|P62987|P62988
• Protein Accession #: NP_001029102.1
• Molecular Weight: 8 kDa