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Datasheets/Manuals | Printable datasheet for UBA52 Antibody (OAAF01979) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human Ubiquitin. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | Ubiquitin antibody detects endogenous levels of total ubiquitin protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:20000 |
Reference | John Hanna, Mol. Cell. Biol., Dec 2003; 23: 9251 - 9261. Matthias Majetschak, Blood, Mar 2003; 101: 1882 - 1890. Fu Shang, J. Biol. Chem., May 2005; 280: 20365 - 20374. Joerg Herrmann, Circ. Res., May 2007; 100: 1276 - 1291. |
Gene Symbol | RPS27A|UBA52|UBB|UBC |
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Gene Full Name | ribosomal protein S27a|ubiquitin A-52 residue ribosomal protein fusion product 1|ubiquitin B|ubiquitin C |
Alias Symbols | 40S ribosomal protein S27a;CEP52;CEP80;epididymis luminal protein 112;epididymis secretory protein Li 50;HEL112;HEL-S-50;HMG20;HUBCEP52;L40;polyubiquitin B;polyubiquitin-B;polyubiquitin-C;RPL40;S27A;UBA80;UBC;UBCEP1;UBCEP80;Ubiquitin A-52 residue ribosomal protein fusion product 1;ubiquitin and ribosomal protein S27a;ubiquitin C;ubiquitin carboxyl extension protein 52;ubiquitin carboxyl extension protein 80;ubiquitin-40S ribosomal protein S27a;ubiquitin-52 amino acid fusion protein;ubiquitin-60S ribosomal protein L40;ubiquitin-CEP52;ubiquitin-CEP80. |
NCBI Gene Id | 6233|7311|7314|7316 |
Protein Name | Polyubiquitin-B|Polyubiquitin-C|Ubiquitin-40S ribosomal protein S27a|Ubiquitin-60S ribosomal protein L40 |
Description of Target | Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. |
Uniprot ID | P0CG47|P0CG48|P62979|P62987|P62988 |
Protein Accession # | NP_001029102.1 |
Molecular Weight | 8 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "UBA52 Antibody (OAAF01979)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "UBA52 Antibody (OAAF01979)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "UBA52 Antibody (OAAF01979)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UBA52 Antibody (OAAF01979)"?
This target may also be called "40S ribosomal protein S27a;CEP52;CEP80;epididymis luminal protein 112;epididymis secretory protein Li 50;HEL112;HEL-S-50;HMG20;HUBCEP52;L40;polyubiquitin B;polyubiquitin-B;polyubiquitin-C;RPL40;S27A;UBA80;UBC;UBCEP1;UBCEP80;Ubiquitin A-52 residue ribosomal protein fusion product 1;ubiquitin and ribosomal protein S27a;ubiquitin C;ubiquitin carboxyl extension protein 52;ubiquitin carboxyl extension protein 80;ubiquitin-40S ribosomal protein S27a;ubiquitin-52 amino acid fusion protein;ubiquitin-60S ribosomal protein L40;ubiquitin-CEP52;ubiquitin-CEP80." in publications.
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What is the shipping cost for "UBA52 Antibody (OAAF01979)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "UBA52 Antibody (OAAF01979)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "UBA52 Antibody (OAAF01979)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "8 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UBA52 Antibody (OAAF01979)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RPS27A|UBA52|UBB|UBC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RPS27A|UBA52|UBB|UBC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RPS27A|UBA52|UBB|UBC"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RPS27A|UBA52|UBB|UBC"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RPS27A|UBA52|UBB|UBC"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RPS27A|UBA52|UBB|UBC"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.