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Catalog No: OKCD01014 (Formerly GWB-KBBTH1)
Size:96WELLS
Price: $675.00
SKU
OKCD01014
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Datasheets/ManualsPrintable datasheet for TYMS ELISA Kit (Human) (OKCD01014)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-92%83-96%97-105%80-91%
EDTA plasma(n=5)92-99%87-95%80-105%83-101%
heparin plasma(n=5)99-105%95-102%79-94%85-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidylate Synthetase (TYMS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidylate Synthetase (TYMS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidylate Synthetase (TYMS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thymidylate Synthetase (TYMS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-9993
EDTA plasma(n=5)87-9591
heparin plasma(n=5)86-9790
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidylate Synthetase (TYMS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidylate Synthetase (TYMS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TYMS Microplate96 Wells (12 x 8 Well Strips)
TYMS Lyophilized Standard2 x 20 ng
100X Biotinylated TYMS Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thymidylate Synthetase (TYMS). No significant cross-reactivity or interference between Thymidylate Synthetase (TYMS) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTYMS
Gene Full Namethymidylate synthetase
Alias SymbolsHST422;thymidylate synthase;TMS;TS;TSase.
NCBI Gene Id7298
Protein NameThymidylate synthase
Description of TargetContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Uniprot IDP04818
Protein Accession #NP_001062
Nucleotide Accession #NM_001071.2
Protein Size (# AA)313
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