TXNDC4 Antibody (OAEB01722)

Catalog No: OAEB01722 (Formerly GWB-D559B2)

Size:100UG

OAEB01722 (0.2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB01722 (0.2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ERP44
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Peptide Sequence: C-EQKDSDNYRVFER
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 47kDa band observed in lysates of cell line K562 (calculated MW of 47.0kDa according to NP_055866.1). Recommended concentration: 0.2-0.6ug/ml.
  An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Anelli T, Ceppi S, Bergamelli L, Cortini M, Masciarelli S, Valetti C, Sitia R.; Sequential steps and checkpoints in the early exocytic compartment during secretory IgM biogenesis.; EMBO J. 2007 Oct 3;26(19):4177-88. Epub 2007 Sep 6.; Pubmed 17805346

Target Info

• Gene Symbol: Erp44|ERP44
• Gene Full Name: endoplasmic reticulum protein 44
• Alias Symbols: 1110001E24Rik;AI849526;AL033348;BWK4;endoplasmic reticulum resident protein 44;endoplasmic reticulum resident protein 44 kDa;endoplasmic reticulum resident protein 44kDa;endoplasmic reticulum resident protein ERp44;epididymis secretory sperm binding protein;ER protein 44;PDIA10;protein disulfide isomerase family A, member 10;thioredoxin domain containing 4 (endoplasmic reticulum);thioredoxin domain-containing protein 4;Txndc;Txndc4.
• NCBI Gene Id: 23071|298066|76299
• Protein Name: Endoplasmic reticulum resident protein 44
• Uniprot ID: Q9BS26
• Protein Accession #: NP_055866.1
• Molecular Weight: 47