Catalog No: OKEH04281
Size:96T
Price: $525.00
SKU
OKEH04281
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Tubb3 ELISA Kit (Mouse) (OKEH04281) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tubb3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tubb3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tubb3 captured in the well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.6% (n = 20)
Mean Inter-assay CV%: <= 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Tubb3 Microplate96 Wells (12 x 8 Well Strips)
Tubb3 Standard2 x 2 ng
Sample Diluent1 x 20 mL
Tubb3 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Mouse Tubulin beta-3 chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTubb3
Gene Full Nametubulin, beta 3 class III
Alias Symbols3200002H15Rik;betaIII-tubulin;M(beta)3;M(beta)6;tubulin beta-3 chain.
NCBI Gene Id22152
Protein NameQ9ERD7
Description of TargetTubulin beta-3 chain
Uniprot IDhttps://www.uniprot.org/uniprot/Q9ERD7
Protein Accession #NP_075768.1
Nucleotide Accession #NM_023279.2
Protein Size (# AA)450
Molecular Weight50 kDa
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