TUBA4A Antibody (OAGA00994)

Catalog No: OAGA00994

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: HeLa
D: Molt-4
10% SDS PAGE
OAGA00994 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
OAGA00994 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using alpha Tubulin 4a(OAGA00994) antibody at 1:100 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin 4a(OAGA00994) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]
  Positive Controls: A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3, *mouse epididymis
  Cellular Localization: Cytoplasm, Cytoskeleton, Microtubule
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 21 and 243 of alpha Tubulin 4a (Uniprot ID#P68366)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.92 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TUBA4A
• Gene Full Name: Tubulin alpha-4A chain
• Alias Symbols: ALS22, TUBA1, H2-ALPHA
• NCBI Gene Id: 7277
• Protein Name: Tubulin alpha-4A chain
• Uniprot ID: P68366
• Protein Accession #: NP_005991.1
• Molecular Weight: 50 kDa