Catalog No: OKEH03527
Size:96T
Price: $625.00
SKU
OKEH03527
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH03527
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TSKU ELISA Kit (Human) (OKEH03527) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TSKU antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TSKU and incubated. The TSKU found in the sample or standards competes with the biotinylated TSKU for limited binding sites on the immobilized anti-human TSKU antibody. Excess unbound biotinylated TSKU and sample or standard TSKU is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TSKU captured in the well and inversely proportional to the amount of TSKU which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 9.4% (n = 20)
Mean Inter-assay CV%: <= 10.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TSKU Microplate96 Wells (12 x 8 Well strips)
TSKU Lyophilized Standard2
Sample Diluent1 x 20 mL
100X TSKU-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.162 ng/mL
SpecificityNatural and recombinant Human Tsukushin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolTSKU
Gene Full Nametsukushi, small leucine rich proteoglycan
Alias SymbolsE2IG4;E2-induced gene 4 protein;leucine rich repeat containing 54;leucine-rich repeat-containing protein 54;LRRC54;TSK;tsukushi;tsukushi homolog;tsukushi small leucine rich proteoglycan homolog;tsukushin.
NCBI Gene Id25987
Protein NameQ8WUA8
Description of TargetTsukushin
Uniprot IDhttps://www.uniprot.org/uniprot/Q8WUA8
Protein Accession #NP_001245139.1
Nucleotide Accession #NM_001258210.1
Protein Size (# AA)353
Molecular Weight38 kDa
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