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Catalog No: OKEH03756
Size:96T
Price: $325.00
SKU
OKEH03756
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TSHB ELISA Kit (Bovine) (OKEH03756) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine TSHB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TSHB and incubated. The found in the sample or standards competes with the biotinylated TSHB for limited binding sites on the immobilized anti-Bovine TSHB antibody. Excess unbound biotinylated TSHB and sample or standard TSHB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TSHB captured in the well and inversely proportional to the amount of TSHB which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Component
ComponentAmount
Anti-TSHB Microplate96 Wells (12 x 8 Well strips)
TSHB Lyophilized Standard2
Sample Diluent1 x 20 mL
100X TSHB-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Bovine Thyrotropin subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTSHB
Gene Full Namethyroid stimulating hormone subunit beta
Alias Symbolsthyroid stimulating hormone beta;thyroid stimulating hormone, beta polypeptide;Thyroid-stimulating hormone subunit beta;thyrotropin beta chain;thyrotropin beta subunit;thyrotropin subunit beta;TSH-B;TSH-beta.
NCBI Gene Id281552
Protein NameP01223
Description of TargetThyrotropin subunit beta
Uniprot IDhttps://www.uniprot.org/uniprot/P01223
Protein Accession #NP_776630.1
Nucleotide Accession #NM_174205.1
Protein Size (# AA)138
Molecular Weight16 kDa
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