Catalog No: OKCD01015 (Formerly GWB-KBBUV1)
Size:96WELLS
Price: $700.00
SKU
OKCD01015
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for TRIP6 ELISA Kit (Mouse) (OKCD01015)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Interactor 6 (TRIP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Receptor Interactor 6 (TRIP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Receptor Interactor 6 (TRIP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thyroid Hormone Receptor Interactor 6 (TRIP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Receptor Interactor 6 (TRIP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Receptor Interactor 6 (TRIP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Trip6 Microplate96 Wells (12 x 8 Well Strips)
Trip6 Lyophilized Standard2 x 20 ng
100X Biotinylated Trip6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.144 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Receptor Interactor 6 (TRIP6). No significant cross-reactivity or interference between Thyroid Hormone Receptor Interactor 6 (TRIP6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTrip6
Gene Full Namethyroid hormone receptor interactor 6
Alias Symbolsthyroid receptor-interacting protein 6;TR-interacting protein 6;TRIP-6;ZRP-1;zyxin-related protein 1.
NCBI Gene Id22051
Protein NameThyroid receptor-interacting protein 6
Description of TargetRelays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor (By similarity).
Uniprot IDQ9Z1Y4
Protein Accession #NP_035769
Nucleotide Accession #NM_011639.3
Protein Size (# AA)480
Write Your Own Review
You're reviewing:TRIP6 ELISA Kit (Mouse) (OKCD01015)
Your Rating
We found other products you might like!