TRIM63 Antibody (OAGA00849)

Catalog No: OAGA00849

Size:100 ul

Sample(30 ug whole cell lysate)
A:Hep G2
10% SDS PAGE
OAGA00849 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Breast ca, using MURF1(OAGA00849) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MURF1 (OAGA00849) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm (By similarity), Nucleus (By similarity)
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1 and 44 of MURF1 (Uniprot ID#Q969Q1)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TRIM63
• Gene Full Name: E3 ubiquitin-protein ligase TRIM63
• Alias Symbols: IRF, SMRZ, MURF1, MURF2, RNF28
• NCBI Gene Id: 84676
• Protein Name: E3 ubiquitin-protein ligase TRIM63
• Uniprot ID: Q969Q1
• Protein Accession #: NP_115977.2
• Molecular Weight: 40 kDa